| # | PLAYER | POSITION | AGE | COLLEGE |
|---|---|---|---|---|
| #0 | William Bradley-King | Defensive End | 28 | Baylor |
| #0 | Renardo Green | Cornerback | 25 | Florida State |
| #0 | Derrick Canteen | Cornerback | 25 | Cincinnati |
| #0 | Isaiah Bolden | Cornerback | 26 | Jackson State |
| #0 | Javon Baker | Wide Receiver | 24 | UCF |
| #0 | Garret Wallow | Linebacker | 27 | TCU |
| #1 | Ricky Pearsall | Wide Receiver | 25 | Florida |
| #2 | Deommodore Lenoir | Cornerback | 26 | Oregon |
| #3 | Brian Robinson Jr. | Running Back | 27 | Alabama |
| #4 | Kurtis Rourke | Quarterback | 25 | Indiana |
| #5 | Demarcus Robinson | Wide Receiver | 31 | Florida |
| #6 | Malik Mustapha | Safety | 23 | Wake Forest |
| #7 | Thomas Morstead | Punter | 40 | SMU |
| #9 | Skyy Moore | Wide Receiver | 25 | Western Michigan |
| #10 | Mac Jones | Quarterback | 27 | Alabama |
| #11 | Brandon Aiyuk | Wide Receiver | 28 | Arizona State |
| #13 | Brock Purdy | Quarterback | 26 | Iowa State |
| #15 | Jauan Jennings | Wide Receiver | 28 | Tennessee |
| #17 | Jordan Watkins | Wide Receiver | 24 | Ole Miss |
| #18 | Eddy Pineiro | Place Kicker | 30 | Florida |
| #18 | Malik Turner | Wide Receiver | 30 | Illinois |
| #19 | Jacob Cowing | Wide Receiver | 25 | Arizona |
| #20 | Upton Stout | Cornerback | 24 | Western Kentucky |
| #23 | Christian McCaffrey | Running Back | 29 | Stanford |
| #25 | Jason Pinnock | Safety | 26 | Pittsburgh |
| #26 | Chase Lucas | Cornerback | 29 | Arizona State |
| #27 | Ji'Ayir Brown | Safety | 26 | Penn State |
| #28 | Darrell Luter Jr. | Cornerback | 26 | South Alabama |
| #29 | Jordan James | Running Back | 22 | Oregon |
| #30 | Eli Apple | Cornerback | 30 | Ohio State |
| #31 | Isaac Guerendo | Running Back | 25 | Louisville |
| #32 | Patrick Taylor Jr. | Running Back | 27 | Memphis |
| #33 | Siran Neal | Cornerback | 31 | Jacksonville State |
| #35 | Tre Tomlinson | Cornerback | 26 | TCU |
| #36 | Marques Sigle | Safety | 23 | Kansas State |
| #41 | Jalen Graham | Linebacker | 25 | Purdue |
| #44 | Kyle Juszczyk | Fullback | 35 | Harvard |
| #45 | Nick Martin | Linebacker | 23 | Oklahoma State |
| #46 | Jon Weeks | Long Snapper | 40 | Baylor |
| #47 | Bryce Huff | Defensive End | 28 | Memphis |
| #48 | Tatum Bethune | Linebacker | 25 | Florida State |
| #49 | Jakob Robinson | Cornerback | 25 | BYU |
| #51 | Robert Beal Jr. | Defensive End | 26 | Georgia |
| #53 | Dee Winters | Linebacker | 25 | TCU |
| #53 | Andrew Farmer | Linebacker | 26 | Lane College |
| #54 | Eric Kendricks | Linebacker | 34 | UCLA |
| #54 | Fred Warner | Linebacker | 29 | BYU |
| #55 | Sebastian Valdez | Defensive Tackle | 23 | Washington |
| #56 | Keion White | Defensive End | 27 | Georgia Tech |
| #56 | Tarron Jackson | Defensive End | 27 | Coastal Carolina |
| #57 | Luke Gifford | Linebacker | 30 | Nebraska |
| #58 | Evan Anderson | Defensive Tackle | 24 | Florida Atlantic |
| #59 | Curtis Robinson | Linebacker | 27 | Stanford |
| #61 | Matt Hennessy | Center | 28 | Temple |
| #62 | Austen Pleasants | Offensive Tackle | 28 | Ohio |
| #63 | Nick Zakelj | Offensive Tackle | 26 | Fordham |
| #64 | Jake Brendel | Center | 33 | UCLA |
| #66 | Drake Nugent | Center | 25 | Michigan |
| #67 | Isaac Alarcon | Offensive Tackle | 27 | N/A |
| #68 | Colton McKivitz | Offensive Tackle | 29 | West Virginia |
| #71 | Trent Williams | Offensive Tackle | 37 | Oklahoma |
| #74 | Spencer Burford | Guard | 25 | UTSA |
| #75 | Brandon Parker | Offensive Tackle | 30 | North Carolina A&T |
| #75 | Connor Colby | Guard | 23 | Iowa |
| #77 | Dominick Puni | Guard | 26 | Kansas |
| #78 | Ben Bartch | Guard | 27 | Saint John's (MN) |
| #81 | Trent Taylor | Wide Receiver | 31 | Louisiana Tech |
| #82 | Brayden Willis | Tight End | 26 | Oklahoma |
| #84 | Kendrick Bourne | Wide Receiver | 30 | Eastern Washington |
| #85 | George Kittle | Tight End | 32 | Iowa |
| #86 | Junior Bergen | Wide Receiver | 23 | Montana |
| #88 | Jake Tonges | Tight End | 26 | California |
| #89 | Luke Farrell | Tight End | 28 | Ohio State |
| #90 | Kevin Givens | Defensive Tackle | 29 | Penn State |
| #91 | Sam Okuayinonu | Defensive Tackle | 27 | Maryland |
| #92 | Jordan Elliott | Defensive Tackle | 28 | Missouri |
| #93 | Kalia Davis | Defensive Tackle | 27 | UCF |
| #94 | Yetur Gross-Matos | Defensive End | 28 | Penn State |
| #95 | Alfred Collins | Defensive Tackle | 24 | Texas |
| #96 | Clelin Ferrell | Defensive End | 28 | Clemson |
| #97 | Nick Bosa | Defensive End | 28 | Ohio State |
| #98 | Mykel Williams | Defensive End | 21 | Georgia |
| #99 | C.J. West | Defensive Tackle | 23 | Indiana |