| # | PLAYER | POSITION | AGE | COLLEGE |
|---|---|---|---|---|
| #0 | McKinnley Jackson | Defensive Tackle | 24 | Texas A&M |
| #0 | Vincent Anthony Jr. | Defensive End | 22 | Duke |
| #0 | Renardo Green | Cornerback | 25 | Florida State |
| #0 | Foster Sarell | Offensive Tackle | 28 | Stanford |
| #0 | Brandon Codrington | Cornerback | 25 | North Carolina Central |
| #1 | Ricky Pearsall | Wide Receiver | 26 | Florida |
| #2 | Deommodore Lenoir | Cornerback | 27 | Oregon |
| #3 | Christian Kirk | Wide Receiver | 29 | Texas A&M |
| #4 | Jacob Cowing | Wide Receiver | 25 | Arizona |
| #5 | Mike Evans | Wide Receiver | 33 | Texas A&M |
| #6 | Malik Mustapha | Safety | 24 | Wake Forest |
| #7 | Corliss Waitman | Punter | 31 | South Alabama |
| #7 | De'Zhaun Stribling | Wide Receiver | 23 | Ole Miss |
| #9 | Brayden Willis | Tight End | 26 | Oklahoma |
| #10 | Mac Jones | Quarterback | 28 | Alabama |
| #11 | Demarcus Robinson | Wide Receiver | 32 | Florida |
| #11 | Brandon Aiyuk | Wide Receiver | 28 | Arizona State |
| #13 | Brock Purdy | Quarterback | 26 | Iowa State |
| #14 | Kurtis Rourke | Quarterback | 26 | Indiana |
| #17 | Jordan Watkins | Wide Receiver | 24 | Ole Miss |
| #18 | Eddy Pineiro | Place Kicker | 31 | Florida |
| #18 | Wesley Grimes | Wide Receiver | 22 | NC State |
| #19 | Deebo Samuel Sr. | Wide Receiver | 30 | South Carolina |
| #20 | Upton Stout | Cornerback | 24 | Western Kentucky |
| #22 | Nate Hobbs | Cornerback | 27 | Illinois |
| #23 | Christian McCaffrey | Running Back | 30 | Stanford |
| #25 | Jack Jones | Cornerback | 28 | Arizona State |
| #26 | Kaelon Black | Running Back | 25 | Indiana |
| #27 | Ji'Ayir Brown | Safety | 26 | Penn State |
| #29 | Jordan James | Running Back | 22 | Oregon |
| #29 | Ashtyn Davis | Safety | 30 | California |
| #29 | Darrick Forrest | Safety | 27 | Cincinnati |
| #31 | Isaac Guerendo | Running Back | 26 | Louisville |
| #33 | Siran Neal | Safety | 32 | Jacksonville State |
| #35 | Ephesians Prysock | Cornerback | 23 | Washington |
| #35 | Sincere McCormick | Running Back | 26 | UTSA |
| #36 | Marques Sigle | Safety | 24 | Kansas State |
| #38 | Jalen Stroman | Safety | 23 | Notre Dame |
| #41 | Jalen Graham | Linebacker | 25 | Purdue |
| #43 | Jakob Robinson | Cornerback | 26 | BYU |
| #43 | Khalil Dinkins | Tight End | 24 | Penn State |
| #44 | Kyle Juszczyk | Fullback | 35 | Harvard |
| #45 | Nick Martin | Linebacker | 24 | Oklahoma State |
| #46 | Jon Weeks | Long Snapper | 40 | Baylor |
| #48 | Tatum Bethune | Linebacker | 25 | Florida State |
| #49 | Garret Wallow | Linebacker | 27 | TCU |
| #50 | Andrew Farmer II | Defensive End | 26 | Lane College |
| #51 | Luke Gifford | Linebacker | 31 | Nebraska |
| #53 | Jaden Dugger | Linebacker | 22 | Louisiana |
| #54 | Fred Warner | Linebacker | 29 | BYU |
| #54 | Khalid Kareem | Defensive End | 28 | Notre Dame |
| #55 | Sebastian Valdez | Defensive Tackle | 24 | Washington |
| #56 | Keion White | Defensive End | 27 | Georgia Tech |
| #57 | Dre Greenlaw | Linebacker | 29 | Arkansas |
| #58 | Jarrett Patterson | Guard | 27 | Notre Dame |
| #59 | Ogbo Okoronkwo | Linebacker | 31 | Oklahoma |
| #59 | Mikail Kamara | Defensive End | 25 | Indiana |
| #62 | Austen Pleasants | Offensive Tackle | 29 | Ohio |
| #63 | Nick Zakelj | Offensive Tackle | 27 | Fordham |
| #64 | Jake Brendel | Center | 34 | UCLA |
| #66 | Drake Nugent | Center | 25 | Michigan |
| #67 | Isaac Alarcon | Offensive Tackle | 28 | N/A |
| #68 | Colton McKivitz | Offensive Tackle | 30 | West Virginia |
| #69 | Enrique Cruz Jr. | Offensive Tackle | 23 | Kansas |
| #71 | Trent Williams | Offensive Tackle | 38 | Oklahoma |
| #74 | Carver Willis | Offensive Tackle | 24 | Washington |
| #75 | Connor Colby | Guard | 23 | Iowa |
| #76 | Vederian Lowe | Offensive Tackle | 27 | Illinois |
| #77 | Dominick Puni | Guard | 26 | Kansas |
| #78 | Brett Toth | Center | 30 | Army |
| #84 | Will Pauling | Wide Receiver | 23 | Notre Dame |
| #85 | George Kittle | Tight End | 33 | Iowa |
| #86 | KhaDarel Hodge | Wide Receiver | 31 | Prairie View A&M |
| #88 | Jake Tonges | Tight End | 27 | California |
| #89 | Jack Stoll | Tight End | 28 | Nebraska |
| #89 | Luke Farrell | Tight End | 29 | Ohio State |
| #90 | Kevin Givens | Defensive Tackle | 29 | Penn State |
| #91 | Sam Okuayinonu | Defensive Tackle | 28 | Maryland |
| #92 | Osa Odighizuwa | Defensive Tackle | 28 | UCLA |
| #93 | Gracen Halton | Defensive Tackle | 22 | Oklahoma |
| #94 | Romello Height | Defensive End | 25 | Texas Tech |
| #95 | Alfred Collins | Defensive Tackle | 25 | Texas |
| #96 | James Thompson Jr. | Defensive Tackle | 24 | Illinois |
| #97 | Nick Bosa | Defensive End | 29 | Ohio State |
| #98 | Mykel Williams | Defensive End | 22 | Georgia |
| #99 | C.J. West | Defensive Tackle | 24 | Indiana |