| # | PLAYER | POSITION | AGE | COLLEGE |
|---|---|---|---|---|
| #0 | Kilian Zierer | Offensive Lineman | 27 | Auburn |
| #0 | Eli Apple | Cornerback | 30 | Ohio State |
| #0 | Adrian Martinez | Quarterback | 25 | Kansas State |
| #0 | Sincere McCormick | Running Back | 25 | UTSA |
| #0 | Renardo Green | Cornerback | 24 | Florida State |
| #0 | Kendrick Bourne | Wide Receiver | 30 | Eastern Washington |
| #1 | Ricky Pearsall | Wide Receiver | 25 | Florida |
| #2 | Deommodore Lenoir | Cornerback | 25 | Oregon |
| #3 | Brian Robinson Jr. | Running Back | 26 | Alabama |
| #4 | Kurtis Rourke | Quarterback | 24 | Indiana |
| #5 | Demarcus Robinson | Wide Receiver | 31 | Florida |
| #6 | Malik Mustapha | Safety | 23 | Wake Forest |
| #6 | Skyy Moore | Wide Receiver | 25 | Western Michigan |
| #7 | Thomas Morstead | Punter | 39 | SMU |
| #7 | Malik Turner | Wide Receiver | 29 | Illinois |
| #9 | Brayden Willis | Tight End | 25 | Oklahoma |
| #10 | Mac Jones | Quarterback | 27 | Alabama |
| #11 | Brandon Aiyuk | Wide Receiver | 27 | Arizona State |
| #13 | Brock Purdy | Quarterback | 25 | Iowa State |
| #14 | Russell Gage Jr. | Wide Receiver | 29 | LSU |
| #15 | Jauan Jennings | Wide Receiver | 28 | Tennessee |
| #17 | Jordan Watkins | Wide Receiver | 23 | Ole Miss |
| #19 | Jacob Cowing | Wide Receiver | 24 | Arizona |
| #20 | Upton Stout | Cornerback | 23 | Western Kentucky |
| #23 | Christian McCaffrey | Running Back | 29 | Stanford |
| #25 | Jason Pinnock | Safety | 26 | Pittsburgh |
| #26 | Chase Lucas | Cornerback | 28 | Arizona State |
| #27 | Ji'Ayir Brown | Safety | 25 | Penn State |
| #28 | Darrell Luter Jr. | Cornerback | 25 | South Alabama |
| #29 | Jordan James | Running Back | 21 | Oregon |
| #31 | Isaac Guerendo | Running Back | 25 | Louisville |
| #32 | Patrick Taylor Jr. | Running Back | 27 | Memphis |
| #33 | Siran Neal | Cornerback | 31 | Jacksonville State |
| #35 | Tre Tomlinson | Cornerback | 25 | TCU |
| #36 | Marques Sigle | Safety | 23 | Kansas State |
| #40 | Derrick Canteen | Cornerback | 24 | Cincinnati |
| #41 | Jalen Graham | Linebacker | 24 | Purdue |
| #44 | Kyle Juszczyk | Fullback | 34 | Harvard |
| #45 | Nick Martin | Linebacker | 22 | Oklahoma State |
| #46 | Jon Weeks | Long Snapper | 39 | Baylor |
| #47 | Bryce Huff | Defensive End | 27 | Memphis |
| #48 | Tatum Bethune | Linebacker | 24 | Florida State |
| #49 | Jakob Robinson | Cornerback | 24 | BYU |
| #51 | Robert Beal Jr. | Defensive End | 25 | Georgia |
| #53 | Dee Winters | Linebacker | 24 | TCU |
| #54 | Fred Warner | Linebacker | 28 | BYU |
| #55 | Sebastian Valdez | Defensive Tackle | 23 | Washington |
| #55 | Drew Moss | Guard | 22 | Colorado State |
| #56 | Tarron Jackson | Defensive End | 27 | Coastal Carolina |
| #56 | Trevis Gipson | Linebacker | 28 | Tulsa |
| #57 | Luke Gifford | Linebacker | 30 | Nebraska |
| #58 | Evan Anderson | Defensive Tackle | 23 | Florida Atlantic |
| #59 | Curtis Robinson | Linebacker | 27 | Stanford |
| #61 | Matt Hennessy | Center | 27 | Temple |
| #62 | Austen Pleasants | Offensive Tackle | 28 | Ohio |
| #63 | Nick Zakelj | Offensive Tackle | 26 | Fordham |
| #64 | Jake Brendel | Center | 33 | UCLA |
| #66 | Drake Nugent | Center | 24 | Michigan |
| #67 | Isaac Alarcon | Offensive Tackle | 27 | N/A |
| #68 | Colton McKivitz | Offensive Tackle | 29 | West Virginia |
| #71 | Trent Williams | Offensive Tackle | 37 | Oklahoma |
| #74 | Spencer Burford | Offensive Tackle | 25 | UTSA |
| #75 | Connor Colby | Guard | 22 | Iowa |
| #77 | Dominick Puni | Guard | 25 | Kansas |
| #78 | Ben Bartch | Guard | 27 | Saint John's (MN) |
| #81 | Trent Taylor | Wide Receiver | 31 | Louisiana Tech |
| #83 | Marquez Valdes-Scantling | Wide Receiver | 30 | South Florida |
| #85 | George Kittle | Tight End | 31 | Iowa |
| #86 | Junior Bergen | Wide Receiver | 22 | Montana |
| #88 | Jake Tonges | Tight End | 26 | California |
| #89 | Luke Farrell | Tight End | 27 | Ohio State |
| #90 | Kevin Givens | Defensive Tackle | 28 | Penn State |
| #91 | Sam Okuayinonu | Defensive Tackle | 27 | Maryland |
| #92 | Jordan Elliott | Defensive Tackle | 27 | Missouri |
| #93 | Kalia Davis | Defensive Tackle | 27 | UCF |
| #94 | Yetur Gross-Matos | Defensive End | 27 | Penn State |
| #95 | Alfred Collins | Defensive Tackle | 23 | Texas |
| #96 | Jordan Jefferson | Defensive Tackle | 24 | LSU |
| #97 | Nick Bosa | Defensive End | 27 | Ohio State |
| #98 | Mykel Williams | Defensive End | 21 | Georgia |
| #99 | CJ West | Defensive Tackle | 23 | Indiana |